TRAP Staining Kit
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TRAP Staining Kit

Tartrate resistant acid phosphatase (TRAP) is the signature enzyme of osteoclasts, which is specifically distributed in osteoclasts. TRAP staining kit is used to visualize osteoclasts in tissues. The basic principle is that under acidic conditions containing tartrate, tartrate-resistant acid phosphatase TRAP can hydrolyze naphthol AS-BI phosphate, and the resulting naphthol AS-BI is combined with pararosaniline solution to form an insoluble wine red substance deposited in the enzyme active site, so AS to realize the color development and localization of tartrate-resistant acid phosphatase.

The basic components of the product: the main components of the reaction buffer are acetic acid buffer and potassium sodium tartrate, pH about 5.0; pararosaniline solution, containing pararosaniline; the main component of sodium nitrite solution is 4% sodium nitrite; AS-BI phosphate substrate solution, the main component is 20 mg/mL naphthol AS-BI phosphate. After staining with this product, TRAP in osteoclasts was Burgundy and localized in the cytoplasm. According to the amount of 300 μL per tissue spot on the slice, the kit can do more than 50 TRAP staining.

 

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Company : Wuhan Servicebio Technology Co., Ltd
Phone : 00862751113188
More Information : View
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TRAP Staining Kit

Tartrate resistant acid phosphatase (TRAP) is the signature enzyme of osteoclasts, which is specifically distributed in osteoclasts. TRAP staining kit is used to visualize osteoclasts in tissues. The basic principle is that under acidic conditions containing tartrate, tartrate-resistant acid phosphatase TRAP can hydrolyze naphthol AS-BI phosphate, and the resulting naphthol AS-BI is combined with pararosaniline solution to form an insoluble wine red substance deposited in the enzyme active site, so AS to realize the color development and localization of tartrate-resistant acid phosphatase.

The basic components of the product: the main components of the reaction buffer are acetic acid buffer and potassium sodium tartrate, pH about 5.0; pararosaniline solution, containing pararosaniline; the main component of sodium nitrite solution is 4% sodium nitrite; AS-BI phosphate substrate solution, the main component is 20 mg/mL naphthol AS-BI phosphate. After staining with this product, TRAP in osteoclasts was Burgundy and localized in the cytoplasm. According to the amount of 300 μL per tissue spot on the slice, the kit can do more than 50 TRAP staining.

 

Saler Company Information

Company : Wuhan Servicebio Technology Co., Ltd
Phone : 00862751113188
More Information : View
Online order registration form